Trevor Huyton

2 Questions 14 Answers 0 Followers

Questions related from Trevor Huyton

Does anyone use stemcell technologies clonacell TCS medium?

I wanted to try clonacell TCS medium rarther than limiting dilution. Does anyone have any experience using it? Also does anyone know how much methycellulose it contains?

03 November 2014 780 0 View

Can someone explain to me if my PAM site is TGG (Trp) and coding how I can make a silent mutation to prevent cleavage of the repair template?

I am looking to make a single mutation but the site I want to use has the sequence aaggactgg PAM tgg mutation agg >GAA or GAG If I use the tgg PAM then the codon can't be changed in the repair...

24 October 2014 3,898 1 View