Does anyone use AAV, lentivirus or adenovirus to induce typical autoimmune diseases model by gene overexpression or knockout?

I want to establish some kind of autoimmune diseases model by gene manipulation with AAV or other viral vector.

04 May 2019 6,001 0 View

Does anyone use AAV, lentivirus or adenovirus to induce typical Lung or Trachea disease model in mice by gene overexpression or knockout?

I want to build mouse lung disease models by gene manipulation with AAV or other vector.

04 May 2019 1,122 0 View

Are there any manufacturers in India that can provide nanoparticles or liposomes for high efficient cell transfection?

I am looking for a manufacturer specializing in transfection reagent raw materials.

04 May 2019 3,272 1 View

What's the best injection method to infect heart with AAV?

I want to infect the mouse heart with AAV9

01 February 2019 8,801 2 View

How to establish AAV-producing stable sf9 cell line with rep and cap gene incorporated into the genome?

I know that there is publicated Bac2.0 system for stable sf9 cell line, but they just use the plasmid transfection and screen the cells with antibiotics. I am wondering whether there is a method...

01 February 2019 2,477 2 View

Is adeno associated virus the best vector for in vivo tissue infection at present? What are the advantages of AAV over lentivirus and adenovirus?

01 February 2019 1,752 6 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View