Does anyone use AAV, lentivirus or adenovirus to induce some kind of typical heart disease models by gene overexpression or knockout?

I want to establish mouse heart disease model by gene manipulation with AAV or other viral vector.

04 May 2019 840 0 View

Does anyone use AAV, lentivirus or adenovirus to induce typical autoimmune diseases model by gene overexpression or knockout?

I want to establish some kind of autoimmune diseases model by gene manipulation with AAV or other viral vector.

04 May 2019 6,001 0 View

Does anyone use AAV, lentivirus or adenovirus to induce typical Lung or Trachea disease model in mice by gene overexpression or knockout?

I want to build mouse lung disease models by gene manipulation with AAV or other vector.

04 May 2019 1,122 0 View

What's the best injection method to infect heart with AAV?

I want to infect the mouse heart with AAV9

01 February 2019 8,801 2 View

How to establish AAV-producing stable sf9 cell line with rep and cap gene incorporated into the genome?

I know that there is publicated Bac2.0 system for stable sf9 cell line, but they just use the plasmid transfection and screen the cells with antibiotics. I am wondering whether there is a method...

01 February 2019 2,477 2 View

Is adeno associated virus the best vector for in vivo tissue infection at present? What are the advantages of AAV over lentivirus and adenovirus?

01 February 2019 1,752 6 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

No title

02 March 2021 3,060 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View