There are sveral way to inject AAV for in vivo heart infection. You could find the detail description on this website: https://www.genemedi.net/i/aav-packaging
1. Tail vein injection is the most used method, but most of AAV would be retained in liver tissue, you will always find that liver is infected better than your target heart.
2. Jugular vein or orbital vein injection may result in better infection of heart than tail vein.
3. The best way to infect heart is in situ injection, but need thoracotomy, which is quite difficult for the beginner.
The injection is very important, but a high titer and excellent purity of AAV would ensure the infection efficiency. Attached is representive heart infecion image with AAV injection.
Genemedi provide provide professional AAV production service, if needed, please visit www.genemedi.net and contact us to [email protected]
There are sveral way to inject AAV for in vivo heart infection. You could find the detail description on this website: https://www.genemedi.net/i/aav-packaging
1. Tail vein injection is the most used method, but most of AAV would be retained in liver tissue, you will always find that liver is infected better than your target heart.
2. Jugular vein or orbital vein injection may result in better infection of heart than tail vein.
3. The best way to infect heart is in situ injection, but need thoracotomy, which is quite difficult for the beginner.
The injection is very important, but a high titer and excellent purity of AAV would ensure the infection efficiency. Attached is representive heart infecion image with AAV injection.
Genemedi provide provide professional AAV production service, if needed, please visit www.genemedi.net and contact us to [email protected]