AAV is so hot in genetherapy area nowadays. I want to infect various mouse tissues to express some genes in my project, actually, lentivirus and adenovirus were also used in earlier studies，but more and more researchers use AAV in animal.
Simon N Waddington Thank you for your information very much. Is there a AAV capsid suitable for kidney infection.
AAV is of course the best vector for tissue infection in vivo. You can find the detail information about AAV on this website: https://www.genemedi.net/i/aav-packaging
Genemedi can provide professional AAV production service.
Listed are several main advantages of AAV 1) Superior biosafety rating. The wild type AAV has not currently been known to cause disease in vivo, and further security of recombinant AAV gene delivery in vivo is ensured after removal of most AAV genome elements. 2) Low immunogenicity. AAV causes a very mild immune response in vivo, lending further support to its apparent lack of pathogenicity during gene delivery. 3) Broad range of infectivity. AAV can infect both dividing and quiescent cells in vivo, allowing gene delivery to a highly diverse range of cell types. 4) Stable expression. Long term gene delivery in vivo can be mediated by AAV.
AAV is preferred because it's well characterised and can be produced at very high titers. Different serotypes can also be used to target different tissues.
The struggle with LV is producing sufficient titers and controlling which cells and tissues are infected if you administer it systemically.
It depends on the tissues you want to transduce, and for how long.
Adenovirus is quite immunogenic, so in immune competent mice expression will usually be transient. Intravenously, Ad5 normally transduces liver, predominantly.
Lentivirus vectors, when administered neonatally, achieve high and stable expression - after i.v. in liver, heart, spleen. Intracranially, in brain. Intramuscularly, in muscle. Etc. Lentivirus vectors usually integrate into the cellular genome, so are not lost through cell division.
AAVs usually have the widest and strongest expression. Some capsids confer very wide tropism, which varies at the age of delivery. Neonatal i.v. injection can even result in efficient delivery to the CNS & PNS.
Simon N Waddington Thank you for your information very much. Is there a AAV capsid suitable for kidney infection.
Hi Yixiong - not sure about AAV which can target the kidney, but a few years ago my former colleagues Laura Denby and Andy Baker engineered an Ad vector for kidney targeting - see Article Development of Renal-targeted Vectors Through Combined In Vi...
As Simon says, Ad's only useful for high level, transient expression in vivo - and quite immunogenic. If you want long term expression, perhaps you should try and engineer the AAV capsid to present the peptides described in the above paper, +/- a kidney specific promoter?