hi
i'm doing lentiviral transduction A549 cell line. during the transduction, i removed the medium containing serum and replace it with serum-free medium and added appropriate amount of virus. after 6 hours, i observe d cells. some of d cells at d centre of 24-well plate looks like dying. the morphology already change. but cells at d corner, dont show any dying symptom. 6 hours post transduction, i add medium containing 10% fbs. can someone explain to me why this condition happened? is it because of my cell starving due to serum-free medium? Thank you =)