Noor hanis abu halim

5 Questions 16 Answers 0 Followers

Questions related from Noor hanis abu halim

Why not all cell expressed GFP even after treatment with puromycin?

Hi, i performed lentivirus transduction on A549 with MOI 5. 72 hours post transduction, i observed the cell under microscope. Almost 70% cell were successfully transduced. Then, i treat the cell...

14 April 2017 5,442 12 View

Has anyone used Lipofectamine 3000 for plasmid transfection in A549 cells?

I will be using Lipofectamine 3000 for plasmid transfection in A549 cells. I plan to test different concentration of plasmid that can give best result. so i decided to test 1ug, 2.5ug and 5ug of...

16 March 2017 7,351 1 View

Does anyone know why cells morphology change (cells look like dying) when i starved cells without serum during transduction?

hi i'm doing lentiviral transduction A549 cell line. during the transduction, i removed the medium containing serum and replace it with serum-free medium and added appropriate amount of virus....

27 February 2017 1,582 13 View

What are the best parameters for electroporation of A549?

I've done several techniques to transfect shRNA-containing plasmid into A549. The techniques that i've done including calcium phosphate, lipo 3000, neon transfection. Now i planned to try...

07 September 2016 8,906 4 View

What is the Puromycin concentration range for BEAS 2B?

Hi, i want to perform transfection on BEAS 2B cell. Thus before i start transfection, i need to perform puromycin killed assay to determine the concentration of puromycin that can killed...

08 August 2016 7,945 3 View