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Hi, i performed lentivirus transduction on A549 with MOI 5. 72 hours post transduction, i observed the cell under microscope. Almost 70% cell were successfully transduced. Then, i treat the cell...
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I will be using Lipofectamine 3000 for plasmid transfection in A549 cells. I plan to test different concentration of plasmid that can give best result. so i decided to test 1ug, 2.5ug and 5ug of...
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hi i'm doing lentiviral transduction A549 cell line. during the transduction, i removed the medium containing serum and replace it with serum-free medium and added appropriate amount of virus....
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I've done several techniques to transfect shRNA-containing plasmid into A549. The techniques that i've done including calcium phosphate, lipo 3000, neon transfection. Now i planned to try...
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