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Questions related from Sajid Khan
I am looking for non-transformed non-tumorigenic human hepatocytes that can be maintained in culture. I found one in ATCC i.e THLE-2 (ATCC® CRL-2706™), but this is a transformed cell line,...
27 August 2019 991 2 View
I am getting IgG heavy and light chain bands at 55 and 25 kDa, respectively, in my Co-IP experiment. These bands are obscuring my proteins of interest which are close to these molecular weights....
21 September 2017 5,972 10 View
The normal platelet count in humans is 150,000-400,000 per uL of blood. Can anybody tell me what is the normal platelet cell count in mice? Does mice suppose to have higher platelet count/uL of...
15 September 2017 5,365 8 View
Is it necessary to record the passage number of suspension cells, for example AML and ALL leukemic cells? If so, how one should record it since like adherent cultures we don't really split them...
11 July 2017 3,842 5 View
I m trying to differentiate 3T3-L1 adipocytes. Can anyone pls tell me, how many days it take for complete differentiation. I differentiated it for 8 days using standard protocol, but i didn't...
22 April 2015 4,562 6 View
