11 Questions 21 Answers 0 Followers
Questions related from Sajid Khan
I am looking for non-transformed non-tumorigenic human hepatocytes that can be maintained in culture. I found one in ATCC i.e THLE-2 (ATCC® CRL-2706™), but this is a transformed cell line,...
27 August 2019 1,045 2 View
I am getting IgG heavy and light chain bands at 55 and 25 kDa, respectively, in my Co-IP experiment. These bands are obscuring my proteins of interest which are close to these molecular weights....
21 September 2017 6,031 10 View
What are the ways (in cell or cell free systems) through which we can experimentally prove that a chemical compound/drug molecule leads to complex formation between two proteins? I did...
19 September 2017 6,022 11 View
The normal platelet count in humans is 150,000-400,000 per uL of blood. Can anybody tell me what is the normal platelet cell count in mice? Does mice suppose to have higher platelet count/uL of...
15 September 2017 5,429 8 View
Is it necessary to record the passage number of suspension cells, for example AML and ALL leukemic cells? If so, how one should record it since like adherent cultures we don't really split them...
11 July 2017 3,895 5 View
I will appreciate if you could share your working experience with conventional/patient-derived xenograft models of acute myelogenous/lymphoblastic leukemia ? How its different from solid tumor...
11 July 2017 3,449 2 View
I am looking for few p53 wild type tumor cells that express high and/or low levels of Mdm2. In the literature I found that liposarcoma cells expresses high levels of Mdm2, but these cells are not...
03 July 2017 7,761 4 View
Does anyone works with or has came across any research article describing the tumor cells which primarily depends on anti-apoptotic/pro-survival protein Bcl-XL for their survival? From Bcl-XL...
03 March 2017 1,510 3 View
While validating the mode of action of a novel anti-cancer compound, we have options of using small interference RNA or pharmacological inhibitors of a particular signaling molecule. In several...
11 January 2016 9,798 6 View
In one of my experiment, I am observing that the animals (Balb/c mice) feeding on high-fat diet are having goosebumps along with a increase in their body weights as compared to control diet-fed...
13 May 2015 2,656 4 View
I m trying to differentiate 3T3-L1 adipocytes. Can anyone pls tell me, how many days it take for complete differentiation. I differentiated it for 8 days using standard protocol, but i didn't...
22 April 2015 4,633 6 View
