How can I open a SXV4 file (SuperCROSS) in SPSS, R or STATA?

Hello everyone, I received some data from Statistics South Africa in an SXV4 format (SuperCROSS) and would like to open it in SPSS (or a similar programme if need be). I have tried opening it...

05 January 2021 2,463 4 View

Which connectivity metric is best to compare changes in habitat over time (PC, PCnum or EC(PC)? Which is best for prioritization of restoration?

I am currently researching a network of species movement between temporary ponds using Least Cost Paths. With Conefor I have calculated the PCnum, EC(PC), and PC (probability of Connectivity, as...

23 November 2020 6,298 3 View

How can I analyse the modal ethnicity of a household and compare this to the ethnicity of the household head (SPSS)?

Hello everyone, I am working with a household survey and have both a person and a household file. I am using the household file for the main part of my analysis but want to explore the household...

21 July 2020 7,716 2 View

Deep Euthetic Solvents, how to prepare?

Hi, everyone. I am starting to work with fractionation of Biomassa by Deep Euthetic Solvents . I came across a DES that is comprised of Lactic acid: Glucose:water (molar ratio 5:1:3), as in ref....

12 March 2020 3,474 3 View

How resilient supy chains are against coronavirus?

Can global supply chains be highly resilient against this global virus?

21 February 2020 3,179 4 View

How can I determine the esterification degree of pectin?

Hello, everyone. Recently, I tried to deteremine by titrimetry the esterification degree (ED) of pectin in our lab. That is usual sutff as one titulate a pectin solution with NaOH till pH 8.5,...

09 October 2018 9,062 7 View

Is it possible to perform a repeated measure M-ANOVA?

Does someone know if I can perform for 1 uV with 4 ordinal categories and >2 interval scaled aVs a repeated measure (M)ANOVA? And if so, could you please recommend me literature/papers to learn...

30 August 2018 6,023 2 View

Are there proofs that interest in sustainability is correlated with research based learning ?

Actually we have a lot of discussions how research based learning pushes the interest in sustainability. In our project laboratory of chemistry it seems as if the students are activated to get...

02 June 2018 908 5 View

Adjusting the pH in a small volume ?

Hallo everyone, the Masters studend i 'am supervising recently encountered a problem related to a protocol for the extraction of lipids from human skin. The protocol involves mechanical mincing,...

13 March 2018 8,184 8 View

Compare the core-genome mutations of lineages and identify genes associated with lineage-specific adaptation: it this a valid approach?

I want to analyze mutations in the core-genome of Xanthomonas oryzae, a bacteria species that comprises two pathovars: pv. oryzae and pv. oryzicola. I already have the core-genome identified...

30 January 2018 1,028 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View