Does anyone know where(online) I can get a complete fish cell culture protocol, specifically for salmon head kidney cell?

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Karthik Raman

Zebrafish retinal explants were cultured in the attached protocol. Perhaps more searches on the site can help you find what you need.

http://www.scigine.com/method.php?ID=16512

Rachel B Breyta

try the American Fisheries Society Blue Book

Alex Datsomor

Thank you all for your answers, I found one!!

Ernesto Rojas

Hi Alex could you link the protocol you found please? I am looking to culture the head kidney cells of stickleback fish for longer than a week.

https://www.fws.gov/policy/aquatichandbook/Volume_2/Chapter10Culture.pdf

Hello Ernesto, please follow the link above. You may also follow the instruction from the providers (sigma) of the SHK-1 cells ( http://www.sigmaaldrich.com/catalog/product/sigma/97111106?lang=en®ion=NO )