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Am currently transfecting Atlantic salmon cell line with pCDNA3.1-nCas9n-Hyg plasmid and wish to enrich the transfected cells via hygromycin selection. Is this possible? Is there an efficient...
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Does anyone know where(online) I can get a complete fish cell culture protocol, specifically for salmon head kidney cell?
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I need to perform an antibiotic selection after transfecting my cells, my questions is: How long can a transient transfection last for me to effectively select the successfully transfected cells.
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