Does anyone know where I can get the ZF4 cell line?

More Sven M. Bergmann's questions See All

Is there anyone who can help us with monoclonal antibodies against carp / koi IgM? May be light chain and / or heavy chain and or junction antibodies?

I need those antobodies for the antibody ELISA, IFAT and WB.

05 June 2018 291 3 View

Who is working with Tilapia Lake Virus (TiLV) and can send perhaps an isolate to my lab?

I have to be prepared if the virus comes up in Europe or Germany.

05 June 2017 8,630 2 View

How can I measure the interferon gamma as protein from cell culture supernatant? Does anyone know an ELISA based system?

We like to see if a fish cell culture produces interferon gamma or somethimg similar after infection with different fish viruses.

03 April 2017 8,368 10 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Emile Alghoul

Hi, you can get it from ATCC.

Link: https://www.atcc.org/Products/All/CRL-2050.aspx

Sven M. Bergmann

Thanks a lot Emile but where it is already running in the lab?

Chuanfu Dong

If you tell me you need ZF4 20 days ago, perhaps you have got it to Germany from our Lab.

What a pity but thanks for the information. I will be back to China in May or June 2017 :-).

Qingling Zhu

How did you get ZF4 at last？ We ordered from ATCC but failed to recovery it. I am in England now, eager to find some rescources.

Thanks to all of you who answered my question. I got the ZF4 now from another source into my lab. It is a very nice cell line and replicate CTV very well. Thanks to Mikolaj Adamek :-).

Best wishes

Sven