How to simulate cyclic voltammetry in Comsol Multiphysics?

Hi! Does anybody know how to Programm a cyclic voltammetry Simulation at Comsol multiphysics for a disk-shaped macroelectrode (2D)? Thank you!

12 January 2021 10,012 3 View

Protein long term storage can have an effect in pH changes?

I have a long term stability study with a protein that as an isoletric point lower than the buffer (Tris buffer), and I am seeing a sustantial drift from the initial pH to 12 month point....

10 November 2020 4,445 3 View

How to embed RGD into PCL membrane?

I am currently working on a project Organ-on-a-chip for my dissertation, and we are using PCL membranes to culture MCF12A breast cells. However, I wanted to add RGD to increase cell adherence to...

14 August 2018 6,823 2 View

Incomplete SDS-PAGE separation, what is the problem?

Sometimes when I run my Glycine SDS-PAGE, the separation of proteins (and ladder, also!) stops by the middle of the gel. This is getting more recurrent, since my last 2 gels have had this problem....

14 April 2014 2,537 3 View

Why does the fluorescence intensity (emission scan experiment) increase after each measurement?

Considering a typical emission scan experiment, why does the intensity increase successively after each measure? PS1: All measurement performed in a solution with constant concentration PS2: Also...

18 March 2014 986 6 View

How can I get a good gel in electrophoresis of milk and fermented whey proteins?

I'm currently trying to analyse the milk and fermented whey's protein profile using SDS-PAGE. I've already tried with Tris-Glycine and Tris-Tricine, but I always keep getting a smeared and poor...

26 February 2014 6,269 3 View

HiPerFect Transfection Reagent for HeLa?

I've been using the HiPerFect reagent from Qiagen to transfect HeLa cells with siRNA. I've optimized the transfection protocol using the number of cells suggested by the reagent guidelines, and it...

08 December 2013 2,969 2 View

Are scientist too cautious about using in-silico design for proteins?

After all the advances in bioinformatics in protein modelling, it feels like scientist still do not trust most of the data generated using in-silico techniques. Do you think this is true? Why? How...

01 January 1970 6,352 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View