I am working on the an experiment where I would like to mimic transcription defects of PEP (Plastid Encoded RNA Polymerase). Tagetitoxin is the most effective inhibitor of PEP and a dose curve would be much easier than making an inducible KD system. Rifampicin works well enough, except that is binds to other RNA polys, and is only binds to PEP core set, that is during etiolation.
Hi Thoms,
I think you'll need to buy some at (https://www.medkoo.com/products/22755).
fred
Just a brief correction on Rifampicin effect. It does not bind another RNAP however it binds all free RNAPs and not those bound and transcribing on the DNA, resulting in residual RNA synthesis.
Thank you both. Frederic, I emailed Medkoo months ago and they never replied to my inquiry.
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