Thoms Payne

3 Questions 5 Answers 0 Followers

Questions related from Thoms Payne

Extraction of mRNA from Polysome Sucrose Gradient purity problems?

I am using polysome sucrose gradient coupled with QPCR in order to investigate the transcription and translation regulation of certain genes under various conditions. *I know I could do ribo-seq...

15 July 2019 7,891 0 View

Does anyone know where I can get some tagetitoxin?

I am working on the an experiment where I would like to mimic transcription defects of PEP (Plastid Encoded RNA Polymerase). Tagetitoxin is the most effective inhibitor of PEP and a dose curve...

14 March 2019 2,262 3 View

What is the minimum amount of sucrose needed in plant media to ensure germination, but not elicit a strong physiological sucrose response?

Fellow Plant Scientists, Many plant labs use sucrose in sterile media to ensure optimum conditions for the plant. Adding sucrose helps to normalize and somewhat synchronize the plants' growth,...

02 July 2018 9,705 12 View