I would like to transfer the F plasmid from E. coli W1485 (K-12 wild type cured from Lambda prophage) into my experimental strain by conjugation. Does anybody know what selection markers are on the F plasmid to select for the conjugants?
Hi, Research gate! I need HELP! I'm trying to prepare nickel nanoparticles for my devices. I use the commonly used method from the article (DOI: 10.1002/adma.201405391). Briefly, 20 mmol...
31 March 2024 7,168 1 View
Has there been any research on managing talents who have excelled in competitions in mathematics, programming, or physics/chemistry/biology? How can we ensure that they make their most valuable...
28 February 2024 6,003 0 View
To what amount can you dilute matrigel? It to inject PDX subcutaneously into mouse. Undiluted it's almost impossible to work with, but overdilution leads to matrigel being incapable to solidify....
23 January 2024 6,510 2 View
Hello. The software which is used to simulate Brownian dynamics was already discussed here...
15 January 2024 7,904 0 View
Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...
24 December 2023 9,368 1 View
Hello, did somebody check what could be the temperature of agarose in a tube when melted in boiling water bath?
15 December 2023 7,037 0 View
I've been trying to isolate a low copy (CEN/ARS) plasmid from yeast that has selective markers for both yeast and bacteria (Trp and Amp). However, when I try to recover the plasmid I can't get any...
20 October 2023 6,749 2 View
I want to examine whether there is a significant difference between two data sets (the same number of participants, continuous variable). One has parametric distribution and another has...
06 September 2023 7,342 0 View
Do I understand correctly that heterozygous Pmel mice have twice as less cells that carry anti-gp100 TCR? This should be right because of the allelic exclusion. If so, can one combine both homo...
05 September 2023 260 1 View
I plan to present my findings on the conference. After that, I want to write a full-text article and submit it to the journal. About 90% of results will be new, while remaining 10% will be from my...
03 September 2023 4,109 3 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...
01 August 2024 6,516 2 View
I have been publishing the Nepalese Journal of Agricultural Sciences (online ISSN 2091-0428; print ISSN 2091-042X) on www.nepjas.com and research gate. I was wondering how I can get an impact...
01 August 2024 1,277 1 View
Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...
01 August 2024 629 3 View
My screen printed electrode CV is given here. For Nerstean one electron reaction the delta E value should be close to 57 mV and ration of Ic/Ia should be close to 1. I did CV of my screen printed...
30 July 2024 4,671 3 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
The threshold voltage (Vth) of a MOS device plays a crucial role for its operation. At the same time, noise is an intrinsic factor. So how noise (flicker or thermal) change with the change in...
29 July 2024 3,246 0 View
Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...
26 July 2024 5,654 3 View
Good morning, I would like to conduct a factor analysis for my work at the municipal level on a panel datae with variables such as income per capita, unmployement rate, percentage employed in a...
25 July 2024 5,104 4 View