I am interested in studying platinum resistance in bladder cancer, specifically , I am looking for an Isogenic pair of bladder cancer cell lines where the parent cell is platinum sensitive and the daughter cell line is platinum resistant? Does anyone know of the existence of such cell lines or is willing to share them? Thank you so much.
Hi Bishoy
I know that Yoon SJ et al established a cisplatin-resistant human bladder cancer cell line, T24/R2, from T24 human bladder cancer cell line (http://www.spandidos-publications.com/or/10/5/1363). You could get the T24 cell line from ATCC but I'm not sure they have the T24R2 also. Maybe the authors of the above mentioned paper will help you by kindly providing the cells if you ask them to.
You could also try to establish the resistance to cisplatin by the stepwise exposure to increasing concentrations of drug. It is feasible and it does not take a very long time. In our laboratory, we established a colorectal cell line resistant to oxaliplatin in 2 -3 months by exposing cells to increasing concentrations of drug.
Dear Bishoy,
You can contact Prof. Walter Berger (University of Vienna; Austria).
He is registered on RG.
Best regards
Robert
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