Does anyone know of a good antibody that recognizes dCas9 , I have used the regular Cas9 antbodies but they do not seem to be working...?

11 November 2016 1 7K Report

Simon Molgaard

Hi Bishoy. Unfortunately i cannot help you find a suitable antibody for your studies. I however hope you will share your experience with these antibodies on www.pAbmAbs.com. Here, scientists can share information about antibodies they use in their research and your experience with these antibodies is very important as you can save time and money for other scientists looking for suitable antibodies against dcas9. Currently, more than 1000 different antibodies have been reviewed by scientists all over the world, and i hope you will contribute as well. Thank you :)

Badges
Science topic

More Bishoy Faltas's questions See All

Cisplatin resistant bladder cancer cell lines

I am interested in studying platinum resistance in bladder cancer, specifically , I am looking for an Isogenic pair of bladder cancer cell lines where the parent cell is platinum sensitive and the...

07 August 2014 6,470 0 View

Does anyone know where I can get cisplatin resistant cell lines?

I am interested in studying platinum resistance in bladder cancer, specifically , I am looking for an Isogenic pair of bladder cancer cell lines where the parent cell is platinum sensitive and the...

07 August 2014 4,570 2 View

Can anyone help with verifying a cancer cell line?

I am receiving a cancer cell line from a collaborator that is not commercially available from ATCC. Is there a way to verify the tissue of origin and the histological subtype? I know you can do...

01 February 2014 1,639 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View