Does anyone know where I can analize stevioside and other steviol glycol?

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Apa pengaruh Dari pengelolaan media sosial sebagai strategi digital marketing pariwisata?

Pengelolaan media sosial telah menjadi bagian penting dalam strategi pemasaran digital pariwisata. Di era digital saat ini, media sosial menyediakan platform yang luas untuk mempromosikan...

04 June 2024 4,349 0 View

Can you recommend Scopus journals, management/ business related, that provide fast acceptance?

02 May 2024 1,927 3 View

How do you sterilize olive oil to gavage to germ free mice?

Hello, I am looking for a protocol to sterilize olive oil that will then be suitable to gavage to germ free mice. Importantly, if it were to be dry-heated, could it pose a fire risk?

28 March 2024 3,760 1 View

I am measuring cell viability of OD-21 cells and my hydrogels through Alamar Blue. When i calculate to get the Blank, i sometimes get a negative?

Why does a negative or a 0 for blank mean? Does this mean that cell viability has stopped?

27 March 2024 9,419 2 View

Does anyone did Nf kB and Phospho NfkB staining flow cytometry?

I'm trying to stain NFKB and Phospho NFKB and I'm not getting any signals at all. I use True-Nuclear™ Transcription Factor Buffer Set. Does anyone have experience with staining this two ? IKB...

27 November 2023 5,822 1 View

What is the best way to extract and purify a specific protien?

I am use to working with cell cultures, but I have a strong feeling that my graduate research will require me to work with different genes and proteins in specific cancer cells. So since I am a...

20 October 2023 5,079 0 View

MN9D Cells are not showing any Dopamine for HPLC analysis. Any suggestions?

We are currently working with MN9D cell line. MN9D cells are mouse midbrain that are Dopaminergic. We do several HPLC analysis on the MN9D cells for over 20 years and have always seen dopa and...

27 September 2023 750 2 View

Is K 562 cell line suitable model for BTKi studies?

Dear colleagues, I would like to assess in vitro the cytotoxic effect and possible Bruton kinase inhibition of a serries of organic compounds as potential therapeutics for the treatment of chronic...

26 July 2023 7,534 3 View

Is the VAS Score free to use?

What VAS pain core is free to use and were can I find proof that it is free. Thanks

23 July 2023 6,538 1 View

Can I customize participant feedback using Qualtrics?

Hi! I hope that someone is able to help me with this. I am planning a social psychology experiment in which I would like to use a masculinity threat prompt. I would like it to be similar to...

22 April 2023 6,679 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View