I have recently tried to isolate potential staphylococcus from waste water samples using Mannitol Salt Agar plates with oxacillin. However after incubation plates had a strange growth on it which
Is the salt concentration of the medium 7.5%? If yes, they are halotolerant bacterial al/yeast colonies other than Staphylococcus.
Hi Scott
could please tell how long the incubation period was? because i doubt that these "weird" growths might be S.aureus but for long period incubation , and also these growths has a mucoid appearance!
Please give more details
Hi guys, thanks for the replies, the salt concentration of the medium was 7.5% and the incubation period was 37oC for 3 days. I had only planned to incubate for 24 hours but was unable to remove plates from incubator until after 3 days
it might be some halotolerant Bacillus species. you should also check the gram reaction and morphology
the colony morphology is similar to Bacillus species. as Kris mentioned it must be a halotolerant Bacillus species. If you didn't inoculate it, obviously its contamination.
I am having same problem while testing of herbal products. The growths are typical of S.aureus. biochemical tests even match but bacterial morphology in gram stain is totally opposite. I am getting gram negative rods in chains and in isolation. I am stumped.
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