I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat coming out, forcing some (maybe is) bubble formation. And may due to the resolving concentration is too high, the gel in the lower edge of the glass plate is difficult to polymerization. Can anyone give me a protocl or some addvice? Thank you !
Dear Haitao Hu, similar questions have been already discussed within RG, please check the following search results link. My Regards
https://www.researchgate.net/search.Search.html?type=question&query=Native%20PAGE
Dear Haitao Hu, similar questions have been already discussed within RG, please check the following search results link. My Regards
https://www.researchgate.net/search.Search.html?type=question&query=Native%20PAGE
https://www.routledgehandbooks.com/doi/10.1201/9780203507438.ch3
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