Bruna Bittencourt

2 Questions 7 Answers 0 Followers

Questions related from Bruna Bittencourt

How can I calculate the concentration of 30nM miRNA from a 5nMol stock?

I have a transfection experiment to be done. The stock miRNA is 5nMol. The manufacturer recommends adding 75uL of sterile water, giving me a concentration of 66.67 uM. How can I have a final...

06 May 2019 4,207 7 View

How to increase the quality of my gDNA extraction from swab of skin cells?

I have to extract genomic DNA from skin cells collected on a swab. I am currently using the PureLink Genomic DNA Kit, and following the protocol for the Mammalian Cells Lysate, however they...

03 April 2019 2,885 3 View