Protocol for PC-3 spheroid (hanging drop) fixation and staining/IF?

Hi everyone, I am currently using the hanging drop method to generate spheroids of PC-3 prostate cancer cells. I would like to know if anyone has a detailed protocol I can use to fix these...

19 February 2020 2,756 1 View

Is it any way to do partition of edges not in same plane in Abaqus?

I am developing a model in Abaqus to simulate the cornea. I now want to partition the solid so as to set the middle part and outside part with different materials. However, I am having difficulty...

11 December 2019 7,645 1 View

Is it necessary to crosslink the RNA and protein while doing RNA binding protein immunoprecipitation?

06 September 2017 9,576 6 View

Reduce hemoglobin in RNA extracted from bird blood for Illumina sequencing

We want to sequence RNA from passerine bird blood to look at host expression. We will need to remove the ribosomal RNA and also the globin RNAs during the library preparation. There is no...

16 July 2017 1,842 4 View

TURBO DNase innactivation using Trizol + chloroform+ isopropanol on RNA suspended in n.f water?

I have extracted RNA from avian blood using TRIzol from ambion. After a 3M sodium acetate / ethanol precipitation, the RIN number reads >8 on the bioanalyzer. I want to treat the RNA with TURBO...

17 May 2017 3,085 5 View

How do I Compare two sets of likert scale data? Perceived and felt emotional responses to music

I am a student who has just conducted a study where I want to compare participants felt and perceived emotional responses when they listen to music. I had 3 conditions which where participants...

11 April 2015 8,969 16 View

Does anyone have experience wtih the DCF-DA from invitrogen lifetec product for ROS measurement by flow?

Or can someone recommend another DCF-DA to use for a flow? I would like to measure ROS after treating the cells with H2O2, like to buy the right DCF-DA, any suggestion will be good.

29 July 2013 832 4 View

How to freeze water into ice without using Liquid Nitrogen or other cryogenic agents?

Well i am preparing ice for my project and to prepare it, we have been using Liq.N2. The current idea is to use a refrigerating unit to maintain the temperature of ice from freezing. But why can't...

11 October 2011 6,985 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View