Does anyone know the expression profile for Sox9 in ATDC5 cell line.?

01 February 2021 0 2K Report

I induced chondrogenesis using 1% Insulin transferrin selenium media and checked using qPCR primers for Sox9. I am facing a few problems as I don't get a consistent trend for the expression of Sox9. Let me know if anybody has tried doing that.

Badges
Science method

More Ankita Jena's questions See All

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Which root in this tap root system, in the picture attached, should be used for root sectioning in order to study the root anatomical details?

There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...

14 February 2021 1,816 3 View

Where can I find the spike protein amino acid sequence of SARS COV-2 variant B.1.1.7?

I need the spike protein amino acid sequence of SARS COV-2 variant B.1.1.7 (also named as VUI 202012/1)

29 December 2020 2,409 3 View

Hi, i am working on PARP inhibitors poorly water soluble but soluble in toxic solvents acetonitrile / DMSO, please suggest nontoxic solvents ?

Drug is poorly water soluble and soluble in solvents like acetonitile , DMSO, mixture of methanol and water but they are toxic. kindly suggest any safe solvent.

18 September 2020 5,128 4 View

How do I conclude if my gene has low expression based on the results obtained?

Hello all, I am working on genes affected in cholesterol metabolism in patients with Coronary Artery Disease and PCSK9 is one such target gene in my study. I have managed to obtain stable results...

13 June 2020 7,074 9 View

Name the journals having highest impact factors in science and pharmaceutical science?

What is the name of the journal in science having highest impact factor? and What is the name of the journal having highest impact factor in pharmaceutical sciences?

03 June 2020 7,054 6 View

What is the institute/organization that provides training for hydroponics development ?

I also want to know the organization working on development of nutrient solution for hydroponics and the organization providing training on it.

02 June 2020 8,901 4 View

How much is the EELS plasmon peak reliable for calculating Fermi energy level for alloys and compounds?

In general the low loss electron energy loss peak contain the information about the electronic structure of materials. From free electron model (Drude Model) the plasmon energy is given by a...

26 May 2020 6,315 3 View

A vaccine effective in monkey against corona virus can be 100% effective in human?

An experimental COVID-19 vaccine protected monkeys from pneumonia and prompted a strong immune response in the animals. Vincent Munster at the National Institute of Allergy and Infectious...

15 May 2020 7,939 6 View

What is the difference between drug loading and Entrapmet Efficiency of drug?

During characterization of Nano-particles, i often get confused between these two terms. Please suggest.

23 April 2020 5,178 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

How to resolve a corrupted .odb file?

Hello. I run my Abaqus simulation in the supercomputer and once it's done, I copied the result (odb file) into my local computer using winSCP. But I faced a problem to read that file (see...

08 February 2021 6,198 3 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

How to compile UDF for parallel processing ?

I am trying to use a UDF for velocity input in the room from a fan. I have created a UDF but when I am trying to load the UDF it shows an error stating that it is not compiled for parallel...

03 February 2021 2,187 3 View

Anyone knows about how to stop pH getting acidic after 7 days formulating the formulation?

i am trying to formulating one formulation but the problem i am facing is after formulating and adjusting it's pH, when i try to check pH after 7 days it is getting down towards the acidic side....

02 February 2021 9,343 3 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View