I got some bands of my compound in TLC. I want to get those compounds for further analysis means for quantification purpose. After scratching the band I have mixed with methanol in a conical flask and mixed throughly with magnetic stirrer for 15 mins. After this i want to filter this solvent. My question is for this filtration which filter paper I will prefer, wheather I will go for whatman 40 or 42 grade or something else.
Depending on the volume, you can simply press a pipette tip to the bottom of the flask and draw up the liquid. This works for a volume of a few milliliters.
Hi Subhrakanta Jena,
The pore size of the filter paper can be compared with the diameter of silica, and the one with a smaller pore size than the diameter of silica can be selected for filtration.