Hello, I've been doing protein purification more in the lab and I want a better understanding of the technique. I've been looking into it on my own but was wondering if anyone knew of a good resource maybe I haven't found yet. Thank you!
Hi Sidney,
I believe you've already read some textbooks (Lehninger, etc). Not sure if you've read this one, but it's really informative:
https://biocev.lf1.cuni.cz/file/282/protein-purification-handbook.pdf
Also, I like to show all the students (or interested people in general) this tool:
http://www.agbooth.com/pp_ajax/
It's very good, you can practice the ideas and the concepts.
Best,
Lorenzo
Dear Sydney Allen
i don't know a single comprensive resource about protein purification but i can provide you some links that i found interesting:
https://www.embl.org/groups/protein-expression-purification/services/protein-purification/#chromatography
more details about IMAC and Ni-NTA are avaialble into the Qiagen Qiaexpressionist
https://kirschner.med.harvard.edu/files/protocols/QIAGEN_QIAexpressionist_EN.pdf
In my blog proteoCool in the protein purification section
https://proteocool.blogspot.com/p/page3-protein-puriifcation-and.html
you can also find some video about IMAC
and in the following video
https://www.blogger.com/video.g?token=AD6v5dzPeA9ws3hcPvrO8fBABN8iFQ0dwI7GIHys9p8dwVtov4dGrKXH9PF91d9bVMYcLxTYY3XJesKt62Y-LsZcaHDCJKZbNW-DLUm_4p5-75Dbj1VZlKTlyyl2aQzf-EPwO3Ac6eBG
beetween minute 7'30 and 8'00 a table with a list of tags used for affinity purification.
This list miss the proteinA, proteinG those are used for affinity purification of antibodies.
i hope that some of this informations may be helpfull to you
good luck
Manuele
best
Manuele
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