http://wolfson.huji.ac.il/purification/

Hi Sidney,

I believe you've already read some textbooks (Lehninger, etc). Not sure if you've read this one, but it's really informative:

https://biocev.lf1.cuni.cz/file/282/protein-purification-handbook.pdf

Also, I like to show all the students (or interested people in general) this tool:

http://www.agbooth.com/pp_ajax/

It's very good, you can practice the ideas and the concepts.

Best,

Lorenzo

Dear Sydney Allen

i don't know a single comprensive resource about protein purification but i can provide you some links that i found interesting:

https://www.embl.org/groups/protein-expression-purification/services/protein-purification/#chromatography

more details about IMAC and Ni-NTA are avaialble into the Qiagen Qiaexpressionist

https://kirschner.med.harvard.edu/files/protocols/QIAGEN_QIAexpressionist_EN.pdf

In my blog proteoCool in the protein purification section

https://proteocool.blogspot.com/p/page3-protein-puriifcation-and.html

you can also find some video about IMAC

and in the following video

https://www.blogger.com/video.g?token=AD6v5dzPeA9ws3hcPvrO8fBABN8iFQ0dwI7GIHys9p8dwVtov4dGrKXH9PF91d9bVMYcLxTYY3XJesKt62Y-LsZcaHDCJKZbNW-DLUm_4p5-75Dbj1VZlKTlyyl2aQzf-EPwO3Ac6eBG

beetween minute 7'30 and 8'00 a table with a list of tags used for affinity purification.

This list miss the proteinA, proteinG those are used for affinity purification of antibodies.

i hope that some of this informations may be helpfull to you

good luck

Manuele

best

Manuele