we are generating a proposal for detection of covid in our state, we need a quote and they have not answered me from NEB
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
I need to install this program
25 July 2024 4,756 0 View
Hello everyone, I'm currently working on calculating the relative levels of mtDNA using qPCR by comparing the Ct values of a mitochondrial gene (nd1) and a nuclear gene. According to my...
16 July 2024 5,508 0 View
Due to the shifting ocean currents, the planet could be thrown off balance, altering the angle of rotation, and even causing the axis to shift, plunging us into chaos. In reality, it doesn't take...
15 July 2024 8,949 1 View
Following https://catniplab.github.io/Tafazoli-LearningControlBrain/ and https://insights.princeton.edu/2020/05/learning-to-control-the-brain/ which commercial technology is available ? Probably...
15 July 2024 4,339 0 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...
30 June 2024 4,107 4 View
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19 June 2024 2,125 3 View
I'been seeing those white spots at the end of my wet-transfer lately. Since the Ponceu Red doesn't stain I guess there isn't any protein. Since it's under the 42kDa mark It isn't a problem since I...
18 June 2024 1,734 0 View
The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...
14 July 2024 1,483 0 View
How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...
10 July 2024 5,040 8 View
Hello. In our laboratory, we are currently conducting Loop-Mediated Isothermal Amplification (LAMP) to detect insert DNA in plasmid vectors. When using the LAMP method, we typically use 8 tubes,...
24 June 2024 645 1 View
I have designed primers for HBB Gene and the template is Whole Genome amplification(WGA) products. The primers work in some samples and in some they don't. I measured the OD of both the samples....
23 June 2024 3,362 2 View
I am conducting a LAMP assay, and while the negative control shows no bands as expected, my positive control samples consistently display only a single band instead of the anticipated ladder-like...
18 May 2024 4,853 3 View
Strain: LBA4404 Growing media: YEB (pH 7) Temperature: 28°C, 200 rpm (overnight culture) Antibiotic use: Kanamycin and Streptomycin
13 May 2024 7,726 2 View
Hi there, My area of research is an aqueous Zinc ion battery and my goal is to find the binding affinity and thermodynamic properties of Zn-ions binding with polyacrylic acid (PAA) by ITC...
30 April 2024 4,171 0 View
I am looking for any research article that demonstrate the effect of different length and Tm of Primers used in LAMP reactions. Does the Bst 2.0 Polymerase have any impact on the Tm of primers?
28 April 2024 5,730 0 View
I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...
28 April 2024 2,113 3 View
I've done the ITC experiments to find the calcium-binding affinity of my protein. I used the built-in program, the MicroCal-ITC analysis software to fit the data. The mode that I used to fit is...
28 April 2024 6,223 3 View