It seems as if most MPNST cell lines have microdeletions or other mutations, I'm particularly interested in tracking down an MPNST cell line that has a point mutation in NF1 as opposed to a larger deletion.
31 December 2014 7,548 6 View
This is perhaps more of a philosophical question - I am wondering for Sanger sequencing of a PCR fragment specifically - but is it necessary to do biological replicates for clean Sanger sequencing...
08 September 2014 8,475 4 View
I'm looking for MPNST cell lines that are strictly from sporadic (non NF associated) MPNSTs to compare to NF associated MPNST. I know of STS-26T and HS-Sch-2, but do others exist? There seem to be...
05 June 2014 6,063 1 View
I'm looking for a NF1 wild-type GBM cell line that doesn't have proteasomal NF1 degradation. In other words I'd like a GBM cell line that has robust NF1 protein content, comparable to non...
02 March 2014 8,530 3 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
02 March 2021 3,060 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...
01 March 2021 9,536 3 View
As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...
01 March 2021 3,199 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View