It seems as if most MPNST cell lines have microdeletions or other mutations, I'm particularly interested in tracking down an MPNST cell line that has a point mutation in NF1 as opposed to a larger deletion.
Are there any off targets of Necrostatin 1 beyond IDO (and the obvious target, RIP1)? We have observed prevention of cell death using a compound we study in combination with Nec1 but have used...
31 December 2014 7,602 6 View
This is perhaps more of a philosophical question - I am wondering for Sanger sequencing of a PCR fragment specifically - but is it necessary to do biological replicates for clean Sanger sequencing...
08 September 2014 8,569 4 View
I'm looking for MPNST cell lines that are strictly from sporadic (non NF associated) MPNSTs to compare to NF associated MPNST. I know of STS-26T and HS-Sch-2, but do others exist? There seem to be...
05 June 2014 6,126 1 View
I'm looking for a NF1 wild-type GBM cell line that doesn't have proteasomal NF1 degradation. In other words I'd like a GBM cell line that has robust NF1 protein content, comparable to non...
02 March 2014 8,592 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View