I'm looking for a NF1 wild-type GBM cell line that doesn't have proteasomal NF1 degradation. In other words I'd like a GBM cell line that has robust NF1 protein content, comparable to non transformed cells.
Hi Chris,
Thanks for the advice - I think we may explore that but we are looking for some solid in vitro models for proof of concept before proceeding to more costly animal models.
Cheers! I'll definitely take a look at these models.
Have you looked at Karen Cichowski's paper? (Proteasomal and Genetic Inactivation of the NF1 Tumor Suppressor in Gliomagenesis, Cancer Cell 2009) She works with several GBM models with WT NF1, including some where she did not see stabilization of NF1 with proteosome inhibitor treatment (figure 3D)
Some of the GBM lines used in that paper do have proteosomal degredation of NF1, but they still express higher levels of NF1 (compared to other non-NF1 negative lines). If you just need "high" levels of NF1 and the proteosome thing is irrelevant to your work, you might want to try including some in a panel and just looking at a western.
Best of luck!
Hi Sara,
Thanks a bunch! I might have to take a look at a panel of cells. I am interested in GBM cells that are essentially "normal" for NF1 that also do not have proteasome mediated degradation. It looks like that figure should provide me with a good starting point. I suppose the main issue is to define "normal," which is a little difficult to do!
Thanks again,
Robert
- Badges
- Science topic