It seems as if most MPNST cell lines have microdeletions or other mutations, I'm particularly interested in tracking down an MPNST cell line that has a point mutation in NF1 as opposed to a larger deletion.
This question explores the impact of public perception of vote buying on voter behavior and electoral participation in Ghana. It seeks to understand how voters' views on the practice of exchanging...
19 July 2024 8,004 0 View
Hello, I am looking for a tool that will do a bulk conversion of references from APA to Harvard. There are over 20 pages of references, so I would like to avoid doing them one at a time. Thank...
07 July 2024 2,003 2 View
Can anyone recommend polyclonal or monoclonal antibodies for Tet methylcytosine dioxygenase 2 (TET2) Tet methylcytosine dioxygenase 3 (TET3) DNA methyltransferase 3 beta (DNMT3B) Ideally, they...
27 June 2024 3,557 1 View
What share is made of steel? Plastics and glass? Aluminum? Iron?
15 June 2024 7,246 2 View
Today is my last day working at Missouri Department of Conservation. I have a new email address, and I don't know how to change it in my profile, so that I will continue to receive ResearchGate...
13 June 2024 8,970 2 View
Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore
04 June 2024 5,688 2 View
Dear researchers and academicians. I am kindly requesting for your contributions to the above question on proxies for profitability of smallholder coffee production. Thanks so much and kind regards.
04 June 2024 722 0 View
I would like to easily track which of my articles are getting reads and which are not without clicking on articles one at a time.
22 May 2024 9,047 3 View
Hello all: Any help appreciated. I am running an intervention with students in a esl environment. I want to compare knowledge and awareness of the topics pre and post intervention. The issue is...
14 April 2024 2,237 2 View
I'm unable to currently continue the work on my thesis due to losing a huge number of cells in wash steps (up to 75% after 2 washes). I'm using 2 cell lines Kasumi-1 and Molm-14. I'm working in...
09 April 2024 3,990 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View