In our lab we ran out of the sheath fluid used in the FACS machine. Does anyone know of another fluid we can use in place of the sheath fluid? We ordered more but it wont be here for another two weeks, and our research needs to continue before then.
Depending on the instrument you may be able to use PBS. Used to run this routinely on a BD FACS Aria without any problems.
Depending on the instrument you may be able to use PBS. Used to run this routinely on a BD FACS Aria without any problems.
PBS or water should be OK for a Calibur or a Canto. It depends on your machine and how it measures how much sheath fluid it has left.
Yes, I think PBS should work without any problems as I have used it in the past
Our LSR (Fortessa) has been running with buffered physiological saline since the start without any problems so I'll second the PBS.
Hi,
flow cytometric analyzers are running fine with PBS (because sheath fluid from BD or Miltenyi or... contains nearly only PBS... and some additionals).
But also: dest. water with sodium azide (0.01%) is working very well and you do not have so many problems with PBS cristallization with the valves or tubing...
and is much cheaper :-).
Sorters do need of course PBS, otherwise you can not do sorting...
Best, Jochen
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