Jared Nasser

4 Questions 1 Answers 0 Followers

Questions related from Jared Nasser

Can anyone recommend a proper method for performing a mixed lymphocyte reaction (MLR) with mouse T and B cells?

I have been using an MLR protocol that hasn't turned up any results as far as cell proliferation over the period of 4-5 days. I am using cell strains from Balb/c and B6 mice as stimulators and...

08 August 2017 5,095 1 View

Can anyone recommend a proper procedure using mitomyocin C to inactivate mice T-cells in order to perform a proper mixed lymphocyte reaction?

This MLR will be done using two different strains of mice that is why it is necessary to inactivate the T-cells, so that possible proliferation can be observed.

10 October 2016 4,622 3 View

Can anyone recommend a proper MLR media for culturing mouse T-cells?

I need to perform a Mixed Lymphocyte Reaction for mouse T-cells

06 June 2016 4,050 1 View

Does anyone know of another fluid we can use in place of the sheath fluid in the FACS machine?

In our lab we ran out of the sheath fluid used in the FACS machine. Does anyone know of another fluid we can use in place of the sheath fluid? We ordered more but it wont be here for another two...

09 September 2015 7,315 6 View