I need to perform a Mixed Lymphocyte Reaction for mouse T-cells
RPMI 1640 supplemented with 10% heat-inactivated FBS, sodium pyruvate (110 mg/l), nonessential amino acids, L-glutamate (1 mM), 2- mercaptoethanol, penicillin and streptomycin and N-acetyl cysteine (Sigma; 10 mmol/l, pH adjusted to 7.2).
Article A kinetic study of the murine mixed lymphocyte reaction by 5...
I have been using an MLR protocol that hasn't turned up any results as far as cell proliferation over the period of 4-5 days. I am using cell strains from Balb/c and B6 mice as stimulators and...
07 August 2017 4,966 1 View
This MLR will be done using two different strains of mice that is why it is necessary to inactivate the T-cells, so that possible proliferation can be observed.
09 October 2016 4,588 3 View
In our lab we ran out of the sheath fluid used in the FACS machine. Does anyone know of another fluid we can use in place of the sheath fluid? We ordered more but it wont be here for another two...
08 September 2015 7,284 6 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
03 March 2021 5,360 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...
24 February 2021 5,072 1 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View