RPMI 1640 supplemented with 10% heat-inactivated FBS, sodium pyruvate (110 mg/l), nonessential amino acids, L-glutamate (1 mM), 2- mercaptoethanol, penicillin and streptomycin and N-acetyl cysteine (Sigma; 10 mmol/l, pH adjusted to 7.2).

Article A kinetic study of the murine mixed lymphocyte reaction by 5...