I have an unknow biological liquid in which I know for sure there is DNA inside. I want to know the sequence.
I can't recommend a company, but I would do :
1. MiSeq sequencing with Nextera Libraries.
2. Profiling with the Ray Meta software using extensive reference sequence databases.
The question is incomplete, I feel. If you just need to know if traces of nucleic acids are present, you can do a reading at abs260/280. If the plan is to identify relatedness of a sample, you need a few comparators such as other members of that relationship matrix (e.g mom, dad, siblings if human, related sub-species if other species). If the idea is to understand broad human ethnicities, genotyping for mitochondrial DNA haplotype is the way to go. See here for common human mtDNA haplotypeshttp://goo.gl/phc9EH. If you want to understand familial relationships, you will have to perform a whole genome SNP genotyping using a SNPchip array like 23andme does. If you need sequences, you can perform a whole genome or exome sequencing of the sample. Many academic core labs as well as private companies do this. Please make sure that you have relevant institutional human subjects mandate to do so.
Are you interested in nucleotide or amino acid sequence?
In case of proteomics, we can help you.
Thanks Frédéric, very clear answer. Dear Joseph, I know there is DNA inside my sample, I know from which organism the biological liquid comes and I want to know the sequence of the DNA present in the liquid, hope you can suggest something. The organism is a yeast. Dear Jens, I am interested in nucleotide sequence, thank you.
You want the entire genome sequence or just interested in some gene sequence?
Dear Dr Termolino,
I am from Science Exchange(a marketplace for scientific experiments). You may access several labs and core facilities through our platform, that can do the sequencing service for you. If you've decided to go for whole genome sequencing or NGS, the following links might help:
https://www.scienceexchange.com/services/whole-genome-seq
https://www.scienceexchange.com/services/illumina-ngs
Best,
Atif
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