I have a RNAeasy plant minikit and lilac columns are depleted, but I have plenty of pink columns. I would like to know if there is a way to avoid using QIAshredder columns.
Add 100 ul of 10% SDS before the 56 degree incubation, chill on ice for 10 minutes, centrifuge for 5 minutes at 13,000g and add the liquid to the pink column then proceed as normal.
Add 100 ul of 10% SDS before the 56 degree incubation, chill on ice for 10 minutes, centrifuge for 5 minutes at 13,000g and add the liquid to the pink column then proceed as normal.
Sometimes I add sterile garnet/sand to the sample with RLT/RLC and vortex for 1-2min to homogenize even more. Spin down ~5min at max before proceeding to pink column.