We have stored some tissue at -80° treated for fixation and ground. This tissue is more than 3 years old. We have the same tissue untreated for fixation and I wonder wich is the best one to use for ChIP-seq.
Does anybody know about any ATAC-seq articles in plants?
10 November 2016 229 6 View
I have a map of a plant BC1 population i have drawn with MAPDISTO software and re-drawn with joinmap4.1 software. Both maps show linkage between two arabidopsis chromosomes probably due to...
09 October 2015 3,880 6 View
I have a RNAeasy plant minikit and lilac columns are depleted, but I have plenty of pink columns. I would like to know if there is a way to avoid using QIAshredder columns. Thank you
01 February 2015 2,858 4 View
I need a precise detection technique to detect bacteria, fungi, plants in soil.
08 September 2013 513 2 View
I have an unknow biological liquid in which I know for sure there is DNA inside. I want to know the sequence.
08 September 2013 9,988 6 View
I have Whatman UNIFILTER microplates used for DNA extraction from Arabidopsis thaliana and I would like to know if there is a way to clean and re-use them (they are very expensive).
04 May 2013 5,225 12 View
Is there an analysis capable of discriminating between taxa DNA in a a soil sample? I was thinking of amplifying specific (and common intra-taxa) rDNA sequences.
03 April 2012 1,633 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View