Does anyone know of a resource listing the average volume of common invertebrate and mammalian cell lines?

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Could anyone tell me the best parameters for transfer of a 7kDa protein? Nitrocellulose vs PVDFat 2uM?

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What fraction of proteins within a cell are of low molecular weight (<15kDa)?

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Does someone know if Ponceau S will stain low molecular weight proteins (~7kD)?

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Will Ponceau S bind proteins of low molecular weight, i.e. less than 10kD?

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Ying Henderson

Attached 3 references for you to give you a general idea about cell volume. Especially, the 3rd reference has comparision from bacteria, yeast, to mammalian cells.

http://www.weizmann.ac.il/plants/Milo/images/humanCellSize120116Clean.pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC494369/pdf/jclinpath00491-0013.pdf

http://kirschner.med.harvard.edu/files/bionumbers/fundamentalBioNumbersHandout.pdf

Dave Unis

Thank you very much Ying.