Dave Unis

7 Questions 19 Answers 0 Followers

Questions related from Dave Unis

Does anyone know of a resource listing the average volume of common invertebrate and mammalian cell lines?

In particular I'm looking for C6/36 and BHK cell volumes.

06 June 2015 5,000 2 View

Could anyone tell me the best parameters for transfer of a 7kDa protein? Nitrocellulose vs PVDFat 2uM?

What is the optimum voltage and ampres? Thank you all in advance.

10 October 2014 601 3 View

What fraction of proteins within a cell are of low molecular weight (<15kDa)?

Any cell type would suffice, just looking for a general reference.

10 October 2014 1,695 4 View

Does anyone know which would promote better transfer for low MW proteins (~7kD)?

I'm trying to transfer a low molecular weight protein from a 15% SDS-PAGE gel to a 0.45um PVDF membrane. I've heard many people suggest using a 0.20 um membrane, but many others suggest that it is...

09 September 2014 334 5 View

Does someone know if Ponceau S will stain low molecular weight proteins (~7kD)?

Unable to see small proteins on my transfer to PVDF membranes.

09 September 2014 2,279 4 View

Will Ponceau S bind proteins of low molecular weight, i.e. less than 10kD?

Ponceau S staining

09 September 2014 6,618 8 View

What fraction of C6/36, or any cell line in general, consists of low molecular weight proteins (less than 10kDa)?

With Ponceau S staining I have not, as of yet been able to see any bands below 10kDa (green band) on a .45um PVDF membrane after 45, 30, or 15 minutes of transfer at 100V and 400mA using a 15%...

09 September 2014 9,470 0 View