Does anyone know of a resource listing the average volume of common invertebrate and mammalian cell lines?

05 June 2015 4,923 2 View

Could anyone tell me the best parameters for transfer of a 7kDa protein? Nitrocellulose vs PVDFat 2uM?

09 October 2014 508 3 View

What fraction of proteins within a cell are of low molecular weight (<15kDa)?

09 October 2014 1,614 4 View

Will Ponceau S bind proteins of low molecular weight, i.e. less than 10kD?

Ponceau S staining

08 September 2014 6,538 8 View

What fraction of C6/36, or any cell line in general, consists of low molecular weight proteins (less than 10kDa)?

With Ponceau S staining I have not, as of yet been able to see any bands below 10kDa (green band) on a .45um PVDF membrane after 45, 30, or 15 minutes of transfer at 100V and 400mA using a 15%...

08 September 2014 9,390 0 View

Does anyone know which would promote better transfer for low MW proteins (~7kD)?

I'm trying to transfer a low molecular weight protein from a 15% SDS-PAGE gel to a 0.45um PVDF membrane. I've heard many people suggest using a 0.20 um membrane, but many others suggest that it is...

08 September 2014 237 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View