Does anyone know is there a column (sort of affinity) for chitin binding protein purification?

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Jeffrey R Brender

There is. It is avaiable from a variety of sources

https://www.neb.com/products/s6651-chitin-resin

Patrawadee Pitakpolrat

Thank you for your reply. However, according to literature, my POI has a chitin binding domain but how can i know will it actually bind to the chitin resin? Are all kind of chitin binding domain bind to chitin? only on type of chitin resin available?

Tengfei Liu

u can just but some chitin resin and incubate your protein with it overnight 4°C, the run a western