Tengfei Liu

6 Questions 6 Answers 0 Followers

Questions related from Tengfei Liu

Why do Raji cell become adherent after 24 hours serum starvation?

Raji cells were cultured in serum-free medium, they all adherent after 24 hours, does anyone know why is this the case?

02 February 2015 2,264 2 View

ERK Phosphorylation problem in hek cells?

I made a stable transfected HEK 293 cell line to expressing my protein, and now I want to use the protein containing supernatant(FCS free) to stimulate the normal HEK 293 cell again, to see...

02 February 2015 462 3 View

I want to check the phosphorylation effect on ERK, does anyone know which protein should I use as positive control?

I want to check the phosphorylation effect on ERK, do anyone know which protein should I use as positive control?

02 February 2015 7,734 2 View

How long do I need to starve the HEK293 cells, if I want to test the phosphorylation effect?

Thank you.

02 February 2015 9,896 5 View

Can I culture Mono Mac 6 with DMEM?

Can I culture Mono Mac 6 with DMEM? Usually we culture mono mac 6 with RPMI, but I want to do some transmigartion experiment and do not want to change medium. Thanks

02 February 2015 9,538 2 View

How can I elute the chitin bind protein from chitin beads?

Thanks in advance.

12 December 2014 6,116 0 View