Wai Tuck Soh

3 Questions 14 Answers 0 Followers

Questions related from Wai Tuck Soh

Protocol to produced selenocysteine incorporated recombinant protein in E.coli?

There is a well-described protocol for the incorporation of selenomethionine into the protein of interest by supplementing excess selenomethionine to the E.coli. However, incorporation of...

06 June 2017 7,394 2 View

How do I remove Pichia pastoris pigment from a purified protein?

I have tried to purify my recombinant protein expressed in a P.pastoris system. However, the pigment binds to the column and is eluted together with my target protein. How can I remove the pigment?

02 February 2014 2,075 6 View

Does anyone know is there a column (sort of affinity) for chitin binding protein purification?

I would like to purify my expressed protein with a chitin binding domain.

09 September 2013 1,935 3 View