I know that you can measure hydroxyproline from tissues which have a greater amount of collagen than cells. However I need to measure hydroxyproline from cells grown from a 12 well plate well.
Also, I noticed that standards when dried at 60 C show a more shallow slope than standards not dried in the oven at 60 C.
The Sigma kit's protocol calls for drying samples at 60 C or an evaporator before doing the assay. If the 60 C oven affects the standards it probably affects the samples as well.
Hi it is possible as I did before it is explained by detail in my previous paper
Hydrogen peroxide: a potent inducer of differentiation of human adipose-derived stem cells into chondrocytes
best
Thanks you for your quick reply. Look forward to reading your paper.
Hi Barbara
It is definitely possible as long as the cells produce enough collagen such that it is in range which the kit can detect. Also you need to remember that the collagen gets secreted into the media.
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