I don't know if it will work, but if you try it, you should do the FITC staining first. The peroxidase staining will probably cause significant oxidative damage to the sample.

Hello, Barbara.

Well, you would get non-specific fluorescent background along with color reaction reagents. It was O.K. with TRITC-sec Ab (red fluorescence). Cells and tissues with a dual reporter gene containing GFP-LacZ gave satisfactory results. In this specific case, we only processed for histochemical localization of LacZ gene expression without hindrance of any fluorescence background, showing clear GFP co- expression.

Best wishes.