We have tried the armenian hamster anti mouse and a secondary for rhodamine to use for fluorescence but it didn't work. We wanted fluorescence so we could double stain with another antibody .
Well, couple of suggestions:
1. Try western blotting, it should work and give you at least some information.
2. Many antibodies (despite claims by the companies) do not work in immunohistochemistry. Many companies such as Santa Cruz claim to have antibodies for everything. My personal experience is that only 50% of their antibodies work. There can be many reasons why the antibodies do not work in immuno.
a) Epitope not available: meaning the site that the antibody bind to is occupied by something else such as another binding protein. Then, you can try antigen retrieval but my opinion is that it is not worth it.
b) different species
c) different tissue meaning different isoforms of proteins etc. Finding one good antibody that really really works in immuno can be a wonderful thing! d) the company is cheating, if some of their antibodies never works you can never figure it out. This is really bad, but you have to remember companies are their to make money. One time I got into a dispute over one particular antibody. Called the company so many times, every time they were saying to change my protocols such as fixative, fixation time, pH etc. After about 20 calls I gave up!
3. You can try other antibodies from some reputable companies such as Sigma.
4. Try polyclonal antibodies.
5. Try different extraction with detergents such as 1% Triton X.
Hope this helps.
Regards
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