Western for alpha Enac in mouse kidney?

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Does anyone know if yoCan you measure hydroxyproline from 125,000 cells?

I know that you can measure hydroxyproline from tissues which have a greater amount of collagen than cells. However I need to measure hydroxyproline from cells grown from a 12 well plate...

05 June 2018 1,272 3 View

We are having a hard time getting antiFCER1a to work on mouse lung for IHC-P?

We have tried the armenian hamster anti mouse and a secondary for rhodamine to use for fluorescence but it didn't work. We wanted fluorescence so we could double stain with another antibody .

09 October 2017 8,549 1 View

Double staining using both HRP and fluorescence on same slide?

I am trying to do double staining on mouse tissue but have not found any good fluorescent method for my target antibody. SO I would like to do both HRP and FITC on the same slide. I have used each...

09 October 2017 3,532 2 View

Does anyone know if you can run normally lysed mouse skin tissue on a western blot and probe for collagen 1?

I have tried a few times and have gotten many bands- probably do to the various isoforms it has. Any suggestions? I read that you need to run a nondenatured gel. However, can you get away with...

01 February 2014 6,133 2 View

How to homogenize mouse skin when doing the sircol assay for soluble collagen?

I am getting variable numbers. I have been sonicating with a probe sonicator after cutting tissue into small 2 mm pieces. After rotating ON in the acetic acid/pepsin buffer(0.5 M ,2.5 mg/ml) my...

31 December 2013 4,759 1 View

Why does the BAPNA tryptase assay give me cloudy results?

I use mast cells lysates in both RIPA or PBS that have been sonicated and centrifuged. Is it the membrane proteins in the lysates?

01 January 1970 5,097 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Russell Linscott

Not a personal use recommendation, but have you looked at mAbs 14E10 or 16E4, available from LSBio ( https://www.lsbio.com/antibodies/scnn1a-antibody-enac-alpha-antibody-clone-16e4-wb-western-ls-c759870/785721?trid=0 )and a few others? We also list other sources for anti-ENaC alpha which you may want to explore: https://www.linscottsdirectory.com/antibodies?q=ENaC%20alpha

Andreas Eisenreich

I would propose

anti-epithelial Sodium Channel alpha antibody (Abcam; cat# ab214192).