Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View

Can authors names in a publish paper influence the visibility of that particular article?

Can the position, grade, number of publication of scientists that authored a paper influence the visibility (acceptance, read, recommendation, citation,....) of an article during the submitting...

14 July 2020 3,011 8 View

A genome wide CRISPR/Cas9 screen for drug resistance in cancer cells?

03 November 2019 7,662 2 View

What kinds of single indicators are appropriate, when we calculate multifunctionality?

A (wide accepted) view is that, when we try to address on the biodiversity-ecosystem function relationships, the multifunctionality index should be calculated by a set of activity indicators,...

02 September 2019 8,370 2 View

How to choose solvent C18 column for separating polar compounds?

I have one polar product with an impurity, which has the similar structure and polarity. I used the Hexane:Ethyl acetate:Methanol in 6:3:1with silical gel. And the difference of Rf is around 0.08,...

06 August 2019 3,572 2 View

What is the more adapted protocol for rat testes using NBF solution?

Hi all, I have found loads of paper to fix rat testes but plenty of protocol variations. I have started to fix some testes in NBF 10% for 24h but it doesn't look enough fixed. Do someone...

07 July 2019 4,721 1 View

How to fabricate/make silica spin column for DNA/RNA extraction?

Dear Friends, Does anyone know how to fabricate/make silica spin column for DNA/RNA extraction? Any books, references and videos are welcome. Thanks in advance. GH Yue

27 June 2019 2,456 3 View

Is possible to remove a co-author who only participated in the reasoning of a paper but who did not write or analyze anaything?

this is an ethical question. We have a conflict with a co-author who stopped its contributions, he only participated at the beginning of the project. I did all data analysis, article writing and...

04 March 2019 3,207 6 View

How to present the complete results of stepwise regression?

The common form is the presentation of regression equation, with the coefficients of determination and p value included in the brackets. But we know that the beta coefficients and sum of squares...

02 March 2019 6,064 6 View

Extracting high frequency component from signal by using pywt in python?

I want to extract the higher frequency component from a signal composed of different frequency components by using the pywt package in python. How do I do this correctly? In order to extract the...

22 January 2019 4,940 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View