I am looking for a specific inhibitor of myosin VI or nonmuscle myosin 2. I am also looking for some generic myosin inhibitors.
I did a quick Google search using "myosin inhibitors" and got a bunch of links to companies that sell myosin inhibitors.
Blebbistatin is an Inhibitor of non-muscle myosin II
This reference popped up in the top hits:
Future Med Chem. 2013 Jan;5(1):41-52. doi: 10.4155/fmc.12.185.
Small-molecule inhibitors of myosin proteins.
Bond LM, Tumbarello DA, Kendrick-Jones J, Buss F.
Blebbistatin, as David mentioned, is a myosin II inhibitor - not just non-muscle myosin II, but muscle myosins as well. It does not inhibit all myosin isoforms, however.
BDM (butanedione monoxame) is another myosin inhibitor. See more on Michael Ostap's RG page: https://www.researchgate.net/publication/10861259_23-Butanedione_monoxime_(BDM)_as_a_myosin_inhibitor
Article 2,3-Butanedione monoxime (BDM) as a myosin inhibitor
What kinds of experiments are we talking about?
In cellular systems Blebbistatin is certainly a commonly used drug. If you do reconstituted in vitro assays you do have other choices as well. In that case you should define your problem more specifically!
Hi Charles, did you find any inhibitors specific to any particular classes of mysoin? I am also looking for inhibtors specific to myosinVI.
A new myosin inhibitor, para-nitroblebbistatin was developed last year (Kepiro et al 2014 Angew Chem, attached). The myosin inhibitory properties are identical to those of blebbistatin; it was tested on M2 blebbing cells, HeLa cells, Dictyostelium cells, zebrafish larvae, starfish embryos, keratocytes, cardiomyocytes - not all results are published. The advantage of the derivative is that para-nitroblebbistatin is not phototoxic, not cytotoxic, not fluorescent, which make it a much more convenient and reliable inhibitor.
Several blebbistatin derivatives have now been published and some are commercially available (e.g. para-aminoblebbistatin or para-nitroblebbistatin) which target myosin 2s, including nonmuscle myosin 2. Recent reviews on the topic are: Article Targeting Myosin by Blebbistatin Derivatives: Optimization a...
andArticle The Medicinal Chemistry and Use of Myosin II Inhibitor ( S )...
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