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Questions related from Charles Fisher
I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...
19 December 2020 5,610 4 View
What is the best way to preserve non-sporulating bacteria for further study?
21 May 2020 5,739 5 View
I have a culture of heterogeneous bacteria that will NOT form endospores. This bacteria was originally isolated in nature and is a complex culture of multiple species. I want to be able to...
21 May 2020 8,567 1 View
I am looking for an Olympus Cx43 model microscope that has been used or was a demo model. I would also settle for other earlier models of Olympus microscopes. I need a 100X objective, a camera...
05 November 2018 4,103 7 View
Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck
02 April 2018 2,978 9 View
I am looking for a dominant negative Wiskott Aldrich Syndrome Protein Mutant. I would prefer if it has an mCherry tag. Our lab would pay for shipping and acknowledge your contributions to any...
15 March 2018 8,190 0 View
Hello, I am attempting to use Quikchange Mutagenesis to introduce a single nucleotide mutation into a circular plasmid. I have run the mutagenesis protocol in a thermal cycler with the following...
30 June 2016 8,174 6 View
I am trying to isolate primary neurons for cell culture and am wondering if there is a difference between using Penn/Strep vs. Gentamicin for my neuronal media?
02 December 2015 4,990 3 View
I am attempting to knockdown a protein in HeLa cells and observe its effect on another protein that I am transfecting into the cells. I am attempting to knockdown (KD) a protein (clathrin) in HeLa...
07 October 2015 446 7 View
How long must HeLa cells be serum starved to induce autophagy? I want to induce autophagy but want a ball park of an idea as to how long they must be placed in serum free media before I can see...
08 May 2015 3,620 3 View
If I would like to knock down one particular gene in HeLa cells, would it be better to use CRISPR or RNAi. I don't want to create a stable cell line, just knock down one gene. What are the...
19 January 2015 4,335 8 View
I am an absolute novice when it comes to analyzing microarray data. I want to troll through the NCBI microarray database and look at a multitude of experiments where my gene of interest was...
15 November 2014 2,180 5 View
I have a membrane protein with a GFP tag on its C-terminus. I have been trying to perform a co-ip on this protein by pulling on the GFP tag, but it has come to my attention that the GFP tag may be...
26 October 2014 7,197 4 View
How long can aliquots of the dynamin inhibitor dynasore be stored in a -20 freezer?
21 November 2013 2,961 0 View
I am trying to replicate a colocalization experiment but I am having no luck. The only difference between the previous experiment and my own is that we are using different transfection reagents:...
19 November 2013 2,302 3 View
I am trying to look for clathrin co-localization with some membrane proteins while they are still on the membrane and before endocytosis. I am using the X22 mouse anti-clathrin monoclonal...
09 November 2013 136 1 View
For cell fixation, is there a big difference between using 2% or 3.7% formaldehyde or paraformaldehyde? Does such a small percentage make a big difference for immunofluorescence?
01 November 2013 5,318 29 View
I want to use a particular antibody for IF. Some protocols say to dissolve the antibody in 10% FBS while other protocols say to dissolve it in 2% BSA. Can the BSA or FBS make a difference for the...
23 October 2013 4,847 7 View
I am looking for a specific inhibitor of myosin VI or nonmuscle myosin 2. I am also looking for some generic myosin inhibitors.
29 August 2013 8,829 6 View
Does anyone know of any cell lines that do not express clathrin? It would probably have to have been a line that was artificially created.
29 July 2013 3,758 1 View
I would like to attempt to isolate hepatocytes from fresh rat livers using trypsin with EDTA. I am wondering if this would be effective or if I should use collagenase.
04 July 2013 1,491 16 View
Every time I attempt a transfection, my cells form into a Swiss-cheese pattern on the coverslips they are coated on. Our lab obtained new HeLa's from ATCC and they grew great and transfected fine...
11 November 2012 6,747 5 View
