26 Questions 13 Answers 0 Followers
Questions related from Charles Fisher
I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...
19 December 2020 5,790 4 View
What is the best way to preserve non-sporulating bacteria for further study?
21 May 2020 5,895 5 View
I have a culture of heterogeneous bacteria that will NOT form endospores. This bacteria was originally isolated in nature and is a complex culture of multiple species. I want to be able to...
21 May 2020 8,678 1 View
I have an Orion Versa Star Pro Multi-Meter and I need to get data off of it into Microsoft Excel. We are currently using Hyper Access as a logging software but this is not meeting our needs. We...
09 January 2019 1,828 0 View
I am looking for an Olympus Cx43 model microscope that has been used or was a demo model. I would also settle for other earlier models of Olympus microscopes. I need a 100X objective, a camera...
05 November 2018 4,178 7 View
Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck
02 April 2018 3,028 9 View
I am looking for a dominant negative Wiskott Aldrich Syndrome Protein Mutant. I would prefer if it has an mCherry tag. Our lab would pay for shipping and acknowledge your contributions to any...
15 March 2018 8,244 0 View
Hello, I am relatively inexperienced with certain molecular biology techniques. I have a gene that has been cloned into a plasmid for me. I would like to leave the N and C Terminal domains intact...
14 November 2016 2,335 2 View
Hello, I am attempting to use Quikchange Mutagenesis to introduce a single nucleotide mutation into a circular plasmid. I have run the mutagenesis protocol in a thermal cycler with the following...
30 June 2016 8,228 6 View
I am trying to isolate primary neurons for cell culture and am wondering if there is a difference between using Penn/Strep vs. Gentamicin for my neuronal media?
02 December 2015 5,040 3 View
I am attempting to knockdown a protein in HeLa cells and observe its effect on another protein that I am transfecting into the cells. I am attempting to knockdown (KD) a protein (clathrin) in HeLa...
07 October 2015 492 7 View
How long must HeLa cells be serum starved to induce autophagy? I want to induce autophagy but want a ball park of an idea as to how long they must be placed in serum free media before I can see...
08 May 2015 3,665 3 View
If I would like to knock down one particular gene in HeLa cells, would it be better to use CRISPR or RNAi. I don't want to create a stable cell line, just knock down one gene. What are the...
19 January 2015 4,374 8 View
I am an absolute novice when it comes to analyzing microarray data. I want to troll through the NCBI microarray database and look at a multitude of experiments where my gene of interest was...
15 November 2014 2,229 5 View
I have a membrane protein with a GFP tag on its C-terminus. I have been trying to perform a co-ip on this protein by pulling on the GFP tag, but it has come to my attention that the GFP tag may be...
26 October 2014 7,260 4 View
How long can aliquots of the dynamin inhibitor dynasore be stored in a -20 freezer?
21 November 2013 3,011 0 View
I am trying to replicate a colocalization experiment but I am having no luck. The only difference between the previous experiment and my own is that we are using different transfection reagents:...
19 November 2013 2,347 3 View
Are there circumstances where proteins can ONLY be seen colocalizing under a confocal microscope and NOT under a standard wide field fluorescence microscope?
12 November 2013 7,740 3 View
I am trying to look for clathrin co-localization with some membrane proteins while they are still on the membrane and before endocytosis. I am using the X22 mouse anti-clathrin monoclonal...
09 November 2013 179 1 View
For cell fixation, is there a big difference between using 2% or 3.7% formaldehyde or paraformaldehyde? Does such a small percentage make a big difference for immunofluorescence?
01 November 2013 5,379 29 View
I want to use a particular antibody for IF. Some protocols say to dissolve the antibody in 10% FBS while other protocols say to dissolve it in 2% BSA. Can the BSA or FBS make a difference for the...
23 October 2013 4,892 7 View
I am looking for a specific inhibitor of myosin VI or nonmuscle myosin 2. I am also looking for some generic myosin inhibitors.
29 August 2013 8,872 6 View
Does anyone know of any cell lines that do not express clathrin? It would probably have to have been a line that was artificially created.
29 July 2013 3,803 1 View
I would like to attempt to isolate hepatocytes from fresh rat livers using trypsin with EDTA. I am wondering if this would be effective or if I should use collagenase.
04 July 2013 1,536 16 View
Is there any special media I should use for cells that I isolate directly from rat livers? Could I use DMEM or F-12 or do I need something specific to keep these cells alive?
04 July 2013 5,478 15 View
Every time I attempt a transfection, my cells form into a Swiss-cheese pattern on the coverslips they are coated on. Our lab obtained new HeLa's from ATCC and they grew great and transfected fine...
11 November 2012 6,794 5 View
