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Questions related from Charles Fisher
I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...
19 December 2020 5,585 4 View
What is the best way to preserve non-sporulating bacteria for further study?
21 May 2020 5,708 5 View
I have a culture of heterogeneous bacteria that will NOT form endospores. This bacteria was originally isolated in nature and is a complex culture of multiple species. I want to be able to...
21 May 2020 8,541 1 View
I am looking for an Olympus Cx43 model microscope that has been used or was a demo model. I would also settle for other earlier models of Olympus microscopes. I need a 100X objective, a camera...
05 November 2018 4,082 7 View
Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck
02 April 2018 2,963 9 View
Hello, I am attempting to use Quikchange Mutagenesis to introduce a single nucleotide mutation into a circular plasmid. I have run the mutagenesis protocol in a thermal cycler with the following...
30 June 2016 8,164 6 View
I am trying to isolate primary neurons for cell culture and am wondering if there is a difference between using Penn/Strep vs. Gentamicin for my neuronal media?
02 December 2015 4,973 3 View
I am attempting to knockdown a protein in HeLa cells and observe its effect on another protein that I am transfecting into the cells. I am attempting to knockdown (KD) a protein (clathrin) in HeLa...
07 October 2015 438 7 View
If I would like to knock down one particular gene in HeLa cells, would it be better to use CRISPR or RNAi. I don't want to create a stable cell line, just knock down one gene. What are the...
19 January 2015 4,323 8 View
I am an absolute novice when it comes to analyzing microarray data. I want to troll through the NCBI microarray database and look at a multitude of experiments where my gene of interest was...
15 November 2014 2,170 5 View
I have a membrane protein with a GFP tag on its C-terminus. I have been trying to perform a co-ip on this protein by pulling on the GFP tag, but it has come to my attention that the GFP tag may be...
26 October 2014 7,189 4 View
I am trying to replicate a colocalization experiment but I am having no luck. The only difference between the previous experiment and my own is that we are using different transfection reagents:...
19 November 2013 2,290 3 View
For cell fixation, is there a big difference between using 2% or 3.7% formaldehyde or paraformaldehyde? Does such a small percentage make a big difference for immunofluorescence?
01 November 2013 5,308 29 View
I want to use a particular antibody for IF. Some protocols say to dissolve the antibody in 10% FBS while other protocols say to dissolve it in 2% BSA. Can the BSA or FBS make a difference for the...
23 October 2013 4,841 7 View
Does anyone know of any cell lines that do not express clathrin? It would probably have to have been a line that was artificially created.
29 July 2013 3,748 1 View
I would like to attempt to isolate hepatocytes from fresh rat livers using trypsin with EDTA. I am wondering if this would be effective or if I should use collagenase.
04 July 2013 1,484 16 View
Every time I attempt a transfection, my cells form into a Swiss-cheese pattern on the coverslips they are coated on. Our lab obtained new HeLa's from ATCC and they grew great and transfected fine...
11 November 2012 6,733 5 View
