Is grain boundary scattering 'always' dominant over surface scattering in grained metal/semiconductor thin films?

Hi all, I was wondering if there is some theoretical insight in the relative contribution to the resistivity of grained thin metal or semiconductor films coming from bulk grain boundary...

18 December 2020 2,969 3 View

Can someone troubleshoot my Pet28a cloning?

I am currently trying to clone a gene of 1500 bp into pet 28a by using the NdeI and XHOI restriction sites. These sites are introduced into my gene by overhang PCR. Restrictions are done for 2...

08 October 2020 5,627 7 View

How to control for industry effects in a comparative case study?

I'm considering to conduct a comparative case study (following Eisenhardt 1989) and need to control for industry effects (as to be able to generalize beyond a single industry). So, I'll select...

13 October 2019 3,908 1 View

Validation of control rate freezer for HSC transplants Planer Integra 750 plus. Slow engraftment of the first 5 patients ?

When validating a new Planer Integra 750 plus we observed very slow engraftment for the first 5 patients compared to other prior patients receiving stem cell grafts frozen with our old Planer as...

01 October 2019 3,743 1 View

What are some unexplored applications of quantum groups to particle physics?

I have been spending some time learning about quantum groups and their representations and am interested in applications to high energy physics. Doing a literature search I found that in the last...

14 March 2016 7,315 5 View

Any suggestions on miRNA validated target gene databases ?

When searching for miRNA validated/experimental target gens what database do people recommend. I have been using TargetScan v 6.2, miRSearch v 3.0, miRTarBase. Recently I have been looking at...

29 April 2015 6,396 6 View

Why do I fail to purify a protein fused to Rat-Fc by protein G?

Hi everybody, I created a recombinant protein consisting of a FLAG tag, my protein of interest and a Rat Fc (IgG2a: Ch1, hinge, Ch2, Ch3). Total size is approximately 60 kDa and the protein is...

30 March 2015 2,283 3 View

Is there any data from cosmological experiments available online?

I am supervising an undergraduate student doing a final year research project in statistics. For her project she would like to look at cosmological parameter estimation using Monte Carlo Markov...

18 March 2015 5,834 7 View

Last week, a German Foundation published a call for applications. I would like to file an application and look for patners from Russia and Ukraine?

Very recently, German Volkswagen Stiftung published its call: "Call for trilateral partnerships/projects of scientists from Ukraine, Russia, and...

11 January 2015 6,065 9 View

Can anyone provide IC50 values of temozolomide at U87 and U251 Glioblastoma cells from cells survival assay (after 72 hours)?

In the literature, there are numerous very high values. However, sometimes the duration of incubation is not mentioned. Thus, I have problems in comparing our values with the literature.

22 January 2014 1,253 9 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View